| International Session (Symposium) 1 (JSGE・JGES) |
| Thu. November 1st 9:00 - 12:00 Room 13: Kobe International Conference Center International Conference Room |
| METABOLOMIC INSIGHT OF CELIAC DISEASE | |||
| Avinash Lomash1, Somesh Kumar1, Seema Kapoor1 | |||
| 1MAMC & Associated LN Hospital, Department of Pediatrics, Genetics Division | |||
|
|||
|
|||
| INTRODUCTION Metabolomic aspects of celiac disease is still not completely explored and there is a big spectrum of altered metabolism associated with the susceptibility of disease or may acts as a predictor of disease severity AIM Study was designed to analyze the baseline and follow status of metabolomic parameters in children with CD and in their FDR MATERIALS & METHOD A total of 152index cases with their 401FDR were recruited.Diagnosis was made according to ESPGAN guidelines.A panel of 10Amino Acid and 29Carnitines were estimated from DBS filter by TMS(LC-MS/MS).A total of 17 plasma AA were analysed by RP-HPLC RESULTS A generalized decreased in acyl carnitines were observed but when correlated Marsh3c,we found that C5:1(0.025±0.003μM/L),C14OH(0.009±0.004μM/L)and C18OH(0.005±0.001μM/L)levels were found significantly low as compared to seronegative FDR(0.06±0.04,0.18±0.001and0.08±0.01μM/L)and controls(0.03-0.13,0.01-0.2and0.02-0.2 μM/L).The levels were increased after first and second six months followup.Levels of Alanine(490.10±50.27μM/L),Phenylalanine(386.58±29.24μM/L)were significantly elevated and citrulline(9.42±2.98μM/L)were significantly low in cases with Marsh3c histopathology when compared with FDR and controls.After the introduction of gluten free diet alanine and phenylalanine levels were decreased and citrulline levels were increased.A correlation was also made between DBS filter paper AA levels and plasma AA levels CONCLUSION Our study concludes that AA may become a significant contributors as metabolomic marker in predicting the severity for the disease.Moreover altered carnitine metabolism may significantly contribute to the fatigability.poor growth and disturbed liver function |
|||
|
Index Term 1: First Degree Relatives (FDR) Index Term 2: Amino Acids (AA) |
|||
| Page Top | |||