Japan Digestive Disease Week 2022
International Session (Symposium)2 (JSH, JSGE)
October 28, 14:30–17:00, Room 8 (Fukuoka International Congress Center 411+412)
IS-S2-1_H
Microbiome correlated with fibrosis markers common to HBV and HCV patients
- 1
- Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine
Background: The mechanism between fibrosis and microbiome has been already reported in mice. However, human microbiome research is needed because the microbiome differs and affected by various factors.
Methods: Between June 2016 and June 2020, fecal samples were obtained from patients positive for HCV antibodies (n=185) or HBs antigen (n=89). Isolatd DNA was analyzed using the Illumina MiSeq sequencing platform targeting for the V3–V4 region of 16s rRNA with Silva databases as references. Spearman's rank correlation coefficient was calculated in each group for two indices (Fib4-index and ALBI score) and the microbiome at genus level. A cutoff value of |r|>0.3, p<0.01 was used.
Result: In the HCV group, fib4-index correlated with only Veillonella (r=0.33, p=4.3×10-6), but no bacteria were identified correlating with ALBI score. In the HBV group, Fib4-index and ALBI score were positively correlated with Veillonella, Streptococcus and Christensenellaceae_R-7_group, and negatively correlated with GCA-900066575 and Bilophila. Two genus each showed positive and negative correlations with only Fib4-index. One genus positive and two genus negative correlations with only ALBI score.
Conclusion: Veillonella positively correlated with Fib4-index in groups of different patient background, and may be associated with fibrosis independent of the etiology. Several common bacteria in the HBV group correlated with Fib4-index and ALBI score.
Methods: Between June 2016 and June 2020, fecal samples were obtained from patients positive for HCV antibodies (n=185) or HBs antigen (n=89). Isolatd DNA was analyzed using the Illumina MiSeq sequencing platform targeting for the V3–V4 region of 16s rRNA with Silva databases as references. Spearman's rank correlation coefficient was calculated in each group for two indices (Fib4-index and ALBI score) and the microbiome at genus level. A cutoff value of |r|>0.3, p<0.01 was used.
Result: In the HCV group, fib4-index correlated with only Veillonella (r=0.33, p=4.3×10-6), but no bacteria were identified correlating with ALBI score. In the HBV group, Fib4-index and ALBI score were positively correlated with Veillonella, Streptococcus and Christensenellaceae_R-7_group, and negatively correlated with GCA-900066575 and Bilophila. Two genus each showed positive and negative correlations with only Fib4-index. One genus positive and two genus negative correlations with only ALBI score.
Conclusion: Veillonella positively correlated with Fib4-index in groups of different patient background, and may be associated with fibrosis independent of the etiology. Several common bacteria in the HBV group correlated with Fib4-index and ALBI score.
- Index Term 1: Microbiome
- Index Term 2: fibrosis