| International Session（Symposium）1(JSGE・JGES・JSH・JSGS・JSGCS) |
| Thu. October 31st 9:00 - 11:30 Room 11: Portopia Hotel South Wing Topaz |
| Benefit of Precision Genomic Analysis Using Molecular Barcoding under Automated Sequencing System | |||
| Miho Sakai1,2, Naoya Kato1, Masao Omata2,3 | |||
| 1Department of Gastroenterology, Graduate School of Medicine, Chiba University, 2Genome Analysis Center, Yamanashi Central Hospital, 3The University of Tokyo | |||
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| Objectives: Since obtaining sufficient tumor tissue from biliary tract cancer (BTC) is difficult, liquid biopsy is expected to complement diagnosis. Recently, molecular barcoding (MB) can detect small burden of variants. The Ion Torrent Genexus system improves efficiency by automating, and enabling more precise analysis through the Resequencing process. In this study, the impact of MB panels on targeted sequencing was compared to conventional, and the advantages of automated sequencing in MB panels were compared to manual. Methods: A total of 112 samples were collected from 20 BTC patients and genomic analysis was performed by conventional panel and MB panel. Genomic analysis of manual and automated sequencing was compared using samples from 12 patients (8 BTC and 4 benign disorders). Results: The number of mutations per patient was significantly higher using the MB panel than using the conventional panel (P = 0.002). Tumor-derived mutations were detected more frequently using the MB panel than the conventional panel (P = 0.023). Manual sequencing did detect errors, while automated with the Resequencing did not. The TAT before and after the equipment upgrade to Genexus was 10 days and 1 day. Conclusion: MB-based liquid biopsy using Genexus is highly sensitive and is the optimal method in the era of precision medicine. |
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Index Term 1: oncogenes Index Term 2: Molecular Diagnostic Techniques |
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